Laboratory of Biochemistry & Molecular Virology

Short Description

The Biochemistry and Molecular Virology Laboratory was established in 2015 (Government Gazette 1673/11-08-2015, issue) and serves the educational, research and development needs of the Department in the field of Biochemistry, and is a body responsible for developing research programs on topics that fall within the laboratory's areas of activity, as well as providing specialized services within the framework of both collaborations and national research infrastructures.

Research Interests

K. Katsani:

The connection of nuclear pore proteins with human diseases.
The nuclear architecture of the model organism D. melanogaster
Protein Expression in Eukaryotic Systems for structural and immunological studies
Photocleavage studies on biomolecules.

P. Chandris:

Dynamics of metabolism in real time – Mitochondrial-Nuclear communication
mRNA dynamics and metabolism in real time in living systems
High-end microscopy, use of genetically encoded fluorescent reporters for the study of molecular interactions and metabolic fluctuations in real time
Omics approaches (proteomics, lipidomics, metabolomics)

Innovative Services

Research Programs

InTechThrace: Integrated Technologies in biomedical research: multilevel biomarker analysis in Thrace” (MIS Code 5047285)
“INSPIRED” (The National Research Infrastructures on Integrated Structural Biology, Drug Screening Efforts and Drug target functional characterization) ΟΠΣ (MIS) 500255 (2018 – 2022).

Publications

Soultas, Stefanos, Meliopoulos Konstantinos, Psomas, George, Katsani Katerina & Fylaktakidou, Konstantina## (2025). Oxime esters on 4-nitrobenzaldehyde and 9,10-anthraquinone-2-carboxaldehyde templates: DNA- and albumin-binding and photocleavage studies. Arkivoc.10.24820/ark.5550190.p012.348.
Ioannides AN, Katsani KR, Ouzounis CA, Promponas VJ. A library of sensitive position-specific scoring matrices for high-throughput identification of nuclear pore complex subunits. NAR Genom Bioinform. 2023 Mar 23;5(1):lqad025. doi: 10.1093/nargab/lqad025. PMID: 36968432; PMCID: PMC10034585.
Vrazas V, Moustafa S, Makridakis M, Karakasiliotis I, Vlahou A, Mavromara P, Katsani KR. A Proteomic Approach to Study the Biological Role of Hepatitis C Virus Protein Core+1/ARFP. Viruses. 2022 Jul 31;14(8):1694. doi: 10.3390/v14081694. PMID: 36016316
Katsani KR, Sakellari D Saliva proteomics updates in biomedicine. J Biol Res (Thessalon). 2019 Dec 12;26:17. doi: 10.1186/s40709-019-0109-7. eCollection 2019 Dec. PMID: 31890650 (review)

Martina Samiotaki, George Panayotou, Chandris P*. Detection of Protein Tyrosine Phosphatase Interacting Partners by Mass Spectrometry (*Corresponding author). Methods Mol Biol 2024:2743:165-180.
Kenworthy C., Liou SH, Chandris P., Wong V., Dziuba P., Liu Wei-Li, Lavis LD, Singer RH, and Coleman RA. “Bromodomains regulate dynamic targeting of the PBAF chromatin-remodeling complex to chromatin hubs” Biophysical Journal, 2022 May 3; 121(9):1738-1752). (Also: bioRxiv 111674; doi: https://doi.org/10.1101/111674).
Chandris P.*, Giannouli CC. Panayotou G. “Imaging approaches for the study of metabolism in real time using genetically encoded reporters” Front Cell Dev Biol. 2022 Jan 18;9:725114. doi: 10.3389/fcell.2021.725114. eCollection 2021 (* Corresponding author).
Guo M., Chandris P., Giannini JP, Trexler AJ, Fischer R., Chen J., Vishwasrao H., Rey-Suarez I., Wu Y., Waterman C., Patterson GH, Upadhyaya A., Taraska J., Shroff H. “Single shot super-resolved total internal reflection fluorescence microscopy” Nature Methods. 2018 Jun;15(6):425-428 [Epub ahead of print]